Yeast Metabolic Engineering -

Yeast Metabolic Engineering

Methods and Protocols

Valeria Mapelli (Herausgeber)

Buch | Softcover
316 Seiten
2016 | Softcover reprint of the original 1st ed. 2014
Humana Press Inc. (Verlag)
978-1-4939-5286-1 (ISBN)
112,34 inkl. MwSt
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

An Overview on Selection Marker Genes for Transformation of Saccharomyces cerevisiae.- Natural and Modified Promoters for Tailored Metabolic Engineering of the Yeast Saccharomyces cerevisiae.- Tools for Genetic Engineering of the Yeast Hansenula polymorpha.- Molecular Tools and Protocols for Engineering the Acid Tolerant Yeast Zygosaccharomyces bailii as a Potential Cell Factory.- Strains and Molecular Tools for Recombinant Protein Production in Pichia pastoris.- Methods for Efficient High Throughput Screening of Protein Expression in Recombinant Pichia pastoris Strains.- Synthetic RNA Switches for Yeast Metabolic Engineering: Screening Recombinant Enzyme Libraries.- Generation of Arming Yeasts with Active Proteins and Peptides via Cell Surface Display System: Cell Surface Engineering, Bio-Arming Technology.- Genetic Engineering of Industrial Saccharomyces cerevisiae Strains Using a Selection/Counter-Selection Approach.- Evolutionary Engineering of Yeast.- Determination of a Dynamic Feeding Strategy for Recombinant Pichia pastoris Strains.- Yeast Metabolomics: Sample Preparation for a GC/MS-Based Analysis.- 13C-Based Metabolic Flux Analysis in Yeast: The Pichia pastoris Case.- Pathway Activity Profiling (PAPi): A Tool for Metabolic Pathway Analysis.- QTL Mapping by Pooled-Segregant Whole Genome Sequencing in Yeast.- Genome Scale Metabolic Models of Yeast, Methods for Their Reconstruction and Other Applications.- Model-Guided Identification of Gene Deletion Targets for Metabolic Engineering in Saccharomyces cerevisiae.- Patents: A Tool to Bring Innovation from the Lab Bench to the Marketplace.

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1152
Zusatzinfo 18 Illustrations, color; 26 Illustrations, black and white; X, 316 p. 44 illus., 18 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Studium 2. Studienabschnitt (Klinik) Humangenetik
Naturwissenschaften Biologie Genetik / Molekularbiologie
Technik Umwelttechnik / Biotechnologie
Schlagworte bioinformatics tools • Genetic Engineering • Hansenula polymorpha • Industrial biotechnology applications • Komagataella spp. • Metabolic models • pichia pastoris • Saccharomyces cerevisiae • yeast metabolism • Yeast metabolome analysis • Zygosaccharomyces bailii
ISBN-10 1-4939-5286-2 / 1493952862
ISBN-13 978-1-4939-5286-1 / 9781493952861
Zustand Neuware
Haben Sie eine Frage zum Produkt?
Mehr entdecken
aus dem Bereich
Eine sehr persönliche Geschichte | Der New York Times-Bestseller

von Siddhartha Mukherjee

Buch | Softcover (2023)
Ullstein Taschenbuch Verlag
21,99
Die revolutionäre Medizin von morgen (Lifespan)

von David A. Sinclair; Matthew D. LaPlante

Buch | Softcover (2020)
DuMont Buchverlag
16,00