Genetic Engineering 3

Plasmid and phage M13 cloning vectors.- I Introduction.- II Bacterial plasmids.- III General purpose amplifiable vectors.- IV Specialized vectors.- V Vectors designed to promote gene expression.- VI Broad host range vectors.- VII Single-stranded DNA phages as cloning vectors.- VIII Summary.- IX Acknowledgements.- X References.- Vectors based on bacteriophage lambda.- I Introduction.- II The lambda genome.- III Replication and maturation of lambda DNA.- IV The recognition of recombinant phages.- V The expression of genes cloned into lambda.- VI Making gene banks with lambda vectors.- VII Conclusions and future developments.- VIII Acknowledgements.- IX References.- Expression of cloned genes in eukaryotic cells using vector systems derived from viral replicons.- I Introduction.- II Criteria for the design of animal virus vectors.- III Systems for the propagation of recombinant DNA molecules as virions.- IV Episomal vectors based on viral genomes.- V Virus-based vectors carrying selectable genes.- VI Vectors for the integration of exogenous DNA into chromosomal DNA.- VII Conclusions.- VIII Acknowledgements.- IX References.- A comprehensive list of cloned eukaryotic genes.- Key.