Expanded Bed Chromatography

Expanded bed adsorption: elution in expanded bed mode.- Monitoring, modeling, and control strategies for expanded-bed adsorption processes.- Factors affecting dispersion in expanded bed chromatography.- Physical and biochemical characterization of a simple intermediate between fluidized and expanded bed contactors.- On-line monitoring of the purification of GST-(His)6 from an unclarified Escherichia coli homogenate within an immobilised metal affinity expanded bed.- The effect of column verticality on separation efficiency in expanded bed adsorption.- Evaluation of the effect of in-bed sampling on expanded bed adsorption.- EBA columns with a distribution system based on local stirring.- Simplified and more robust EBA processes by elution in expanded bed mode.- Cell/adsorbent interactions in expanded bed adsorption of proteins.- Validation issues related to expanded bed technology.- Highly dense beaded sorbents suitable for fluidized bed applications.- Characterization of very dense mineral oxide—gel composites for fluidized-bed adsorption of biomolecules.- Expanded bed adsorption on supermacroporous cross-linked cellulose matrix.- Characterisation of STREAMLINETM Phenyl.- Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins.- A simplified purification procedure of a-lactalbumin from milk using Ca2+-dependent adsorption in hydrophobic expanded bed chromatography.- Expanded bed adsorption as a unique unit operation for the isolation of bacteriocins from fermentation media.- Pilot scale recovery of monoclonal antibodies by expanded bed ion exchange adsorption.- Capture of human Fab fragments by expanded bed adsorption with a mixed mode adsorbent.- Purification of Recombinant Human Serum Albumin. Efficient purification usingSTREAMLINE.- Direct coupling of expanded bed adsorption with a downstream purification step.- Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography.- Development and scale up of a capture step (expanded bed chromatography) for a fusion protein expressed intracellularly in Escherichia coli.- On-line monitoring of glucose and/or lactate in a fermentation process using an expanded micro-bed flow injection analyzer.- Binding assays in heterogeneous media using a flow injection system with an expanded micro-bed adsorption column.- Use of a micro-expanded bed containing immobilised lysozyme for cell disruption in flow injection analysis.- Flow injection analysis of intracellular ?-galactosidase in Escherichia coli cultivations, using an on-line system including cell disruption, debris separation and immunochemical quantification.- Key word Index.- Author Index.