Directed Evolution Library Creation

Error-Prone PCR and Effective Generation of Gene Variant Libraries for Directed Evolution.- Error-Prone Rolling Circle Amplification Greatly Simplifies Random Mutagenesis.- Random Mutagenesis by Error-Prone Pol Plasmid Replication in Escherichia coli.- The Sequence Saturation Mutagenesis (SeSaM) Method.- Generation of Effective Libraries by Neutral Drift.- Site Saturation Mutagenesis.- Iterative Saturation Mutagenesis (ISM): A Powerful Approach to Engineer Proteins by Systematically Simulating Darwinian Evolution.- Generating Targeted Libraries by the Combinatorial Incorporation of Synthetic Oligonucleotides During Gene Shuffling (ISOR).- OmniChange: Simultaneous Site-Saturation of up to Five Codons.- Random Insertional–Deletional Strand Exchange Mutagenesis (RAISE): A Simple Method for Generating Random Insertion and Deletion Mutations.- Transposon-Based Approaches for Generating Novel Molecular Diversity during Directed Evolution.- Restriction Enzyme-Mediated DNA Family Shuffling.- Assembly of Designed Oligonucleotides (ADO): A Useful Tool in Synthetic Biology for Creating High Quality Combinatorial DNA Libraries.- One-Pot, Simple Methodology for Cassette Randomization and Recombination for Focused Directed Evolution (OSCARR).- USER Friendly DNA Recombination (USERec): Gene Library Construction Requiring Minimal Sequence Homology.- ITCHY: Incremental Truncation for the Creation of Hybrid Enzymes.- Generating Random Circular Permutation Libraries.- Probabilistic Methods in Directed Evolution: Library Size, Mutation Rate and Diversity.- The Mutagenesis Assistant Program.- Computational Tools for Designing Smart Libraries.- Computational Tools for Directed Evolution: A Comparison of Prospective and Retrospective Strategies.- Designing Libraries of Chimeric Proteins Using SCHEMA Recombination and RASPP.- Non-Contiguous SCHEMA Protein Recombination.- Engineering Proteins by Reconstructing Evolutionary Adaptive Paths.