Skeletal Development and Repair

Overview of Skeletal Development.- Overview of Skeletal Repair (Fracture Healing and Its Assessment).- Generation of Closed Transverse Fractures in Small Animals.- The Murine Femoral Bone Graft Model and a Semi-Automated Histomorphometric Analysis Tool.- Surgical Induction of Posttraumatic Osteoarthirits.- A Mouse Model of Flexor Tendon Repair.- Cartilage Explant Cultures.- Renal Capsule Transplantations to Assay Skeletal Angiogenesis.- Whole Mount Skeletal Staining.- Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens.- Preparation of Thin Sections from Non-fixed and Undecalcified Hard Tissues Using Kawamoto’s Film Method (2012).- Demineralized Murine Skeletal Histology.- Beta-Galactosidase Staining in the Skeleton.- Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues.- Non-Radioactive In Situ Hybridization on Skeletal Tissue Sections.- Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections.- Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections.- TUNEL Assay on Skeletal Tissue Sections to Detect Apoptosis.- Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme.- Isolation and Culture of Murine Primary Chondrocytes.- Bone Marrow Stromal Cell Assays – In Vitro and In Vivo.- Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts.- RANKL-BasedOsteoclastogenic Assays from Murine Bone Marrow Cells.- Hematopoietic Stem Cell Cultures and Assays.