Optical Techniques in Regenerative Medicine

Stephen P. Morgan, PhD, is a professor in biomedical engineering at the University of Nottingham. Since 1992, he has been developing novel optical techniques for imaging and spectroscopy of tissues, with a particular interest in microcirculation. The imaging devices have been used in clinical studies and have led to a blood flow imager marketed under license to Moor Instruments. His other research interests include developing novel methods of imaging and sensing in regenerative medicine for in vivo and in vitro tissues. Felicity R.A.J. Rose, PhD, is an associate professor in tissue engineering at the University of Nottingham. Her research focuses on developing materials that can control stem and differentiated cell behavior during the tissue regeneration process to develop in vivo-like in vitro models for safety, toxicity, and drug screening applications and ultimately tissues for transplantation. Stephen J. Matcher, PhD, is a reader in biomedical engineering at the University of Sheffield. Since 1992, he has conducted research on the use of optical techniques to characterize tissue structure and function. He has developed optical and near-infrared spectroscopy as a tool for the non-invasive measurement of tissue oxygenation. His current research focuses on the use of optical coherence tomography and non-linear microscopy to characterize the structure of native biological tissues and tissue-engineered replacements.

Introduction and Background: The Role of Optical Techniques in Regenerative Medicine. Optical Microscopy in Regenerative Medicine: Fluorescence Microscopy. Second-Harmonic Generation. Two-Photon Polymerization for Tissue-Engineered Scaffold Fabrication. In Situ Microscopy. Optical Spectroscopy in Regenerative Medicine: Light Scattering as Polarization Spectroscopy. Fluorescence Spectroscopy. Raman Spectroscopy. Optical Tomography in Regenerative Medicine: Optical Transillumination and Projection Tomography for Multidimensional Mesoscopic Imaging. Optical Coherence Tomography: Overview and Applications. Application of Polarization-Sensitive OCT and Doppler OCT in Tissue Engineering. Photoacoustic Tomography. Ultrasound-Modulated Optical Tomography. Optical Macroscopic Imaging in Regenerative Medicine: Macroscopic Imaging in Regenerative Medicine. Bioluminescence Imaging. Index.