Colloidal Nanoparticles in Biotechnology

Abdelhamid Elaissari , PhD, DEA, is Director of Research at the National Center for Scientific Research (CNRS) in Lyon, France. A partial list of his current research interests includes functionalization of complex colloidal systems, physical chemistry and colloidal characterization of colloidal particles, adsorption, desorption, covalent grafting, and specific immobilization of biomolecules and polyelectrolytes onto colloidal particles.

About the Editor vii Preface ix Contributors xi 1. Reactive Nanocolloids for Nanotechnologies and Microsystems 1 Christian Pichot 2. Nanoparticles Comprising pH/Temperature-Responsive Amphiphilic Block Copolymers and Their Applications in Biotechnology 31 Peihong Ni 3. Evolution in Malaria Disease Detection: From Parasite Visualization to Colloidal-Based Rapid Diagnostic 65 Duangporn Polpanich, Pramuan Tangboriboonrat, and Abdelhamid Elaissari 4. Antigen-Antibody Interactions Detected by Quasi-Elastic Light Scattering and Electrophoretic Mobility Measurements - A New Concept for Latex Immunodiagnostic Test 95 Teresa Basinska and Stanislaw Slomkowski 5. Biospecific Reactions by Affinity Latexes from Diagnostics to Multiplex Assay 117 Haruma Kawaguchi 6. Fluorescent Colloidal Particles as Detection Tools in Biotechnology Systems 133 Igor Nabiev, Alyona Sukhanova, Mikhail Artemyev, and Vladimir Oleinikov 7. Biofunctionalized Magnetic Micro/Nanoparticles for Biosensing Technologies 169 Nicole Jaffrezic-Renault, Claude Martelet, Yann Chevolot, and Jean-Pierre Cloarec 8. Colloids, Biotechnology, and Microfluidics 199 Cecile Cottin-Bizonne and Rosaria Ferrigno 9. Gas Sensors Based on Ultrathin Films of Conducting Polymers and Nanocomposites 223 Manoj K. Ram, Ozlem Yavuz, and Matt Aldissi 10. Force Measurements between Emulsion Droplets As a New Tool for Medical Diagnostics 247 Abdelhamid Elaissari, Celine Genty, and Olivier Mondain-Monval 11. Particles for Protein Analysis in Microfluidic Systems 275 Anne Le Nel, Myriam Taverna, and Jean-Louis Viovy 12. Semicarbazide/ a -oxo Aldehyde Site-Specific Ligation Chemistry: From Peptide Microarrays to the Micropatterning of Polycarbonate or Titanium Oxide Using Silica Nanoparticles 299 Ouafaa El-Mahdi, Vianney Souplet, Olivier Carion, Clement Roux, Jean-Michel Garcia, Celine Maillet, Christophe Olivier, Oleg Melnyk and Jean-Olivier Durand 13. Electrical Detection of Antibodies from Human Serum Based on the Insertion of Gold-Labeled Secondary Antibodies into Microor Nanogaps 329 Lionel Marcon, Didier Stie'venard, and Oleg Melnyk Index 353