PCR Cloning Protocols

Performing and Optimizing PCR.- Polymerase Chain Reaction.- Computer Programs for PCR Primer Design and Analysis.- Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming.- XL PCR Amplification of Long Targets from Genomic DNA.- Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments.- Long Distance Reverse-Transcription PCR.- Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues.- GC-Rich Template Amplification by Inverse PCR.- PCR Procedure for the Isolation of Trinucleotide Repeats.- Methylation-Specific PCR.- Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads.- Cloning PCR Products.- Cloning PCR Products.- Using T4 DNA Polymerase to Generate Clonable PCR Products.- Enzyme-Free Cloning of PCR Products and Fusion Protein Expression.- Directional Restriction Site-Free Insertion of PCR Products into Vectors.- Autosticky PCR.- A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses.- Mutagenesis and Recombination.- PCR Approaches to DNA Mutagenesis and Recombination.- In-Frame Cloning of Synthetic Genes Using PCR Inserts.- Megaprimer PCR.- PCR-Mediated Recombination.- PCR Method for Generating Multiple Mutations at Adjacent Sites.- A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes.- PCR Screening in Signature-Tagged Mutagenesis of Essential Genes.- Staggered Extension Process (StEP) In Vitro Recombination.- Random Mutagenesis by Whole-Plasmid PCR Amplification.- Cloning Unknown Neighboring DNA.- PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants.-Long Distance Vectorette PCR (LDV PCR).- Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA (“Cold-Start Method”).- Inverse PCR.- 31 Inverse PCR.- Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers.- The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR.- Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis.- Isolation of Large-Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR.- A #x201C;Step Down#x201D; PCR-Based Technique for Walking Into and the Subsequent Direct-Sequence Analysis of Flanking Genomic DNA.- Library Construction and Screening.- Use of PCR in Library Screening.- Cloning of Homologous Genes by Gene-Capture PCR.- Rapid and Nonradioactive Screening of Recombinant Libraries by PCR.- Rapid cDNA Cloning by PCR Screening (RC-PCR).- Generation and PCR Screening of Bacteriophage ? Sublibraries Enriched for Rare Clones (the “Sublibrary Method ”).- PCR-Based Screening for Bacterial Artificial Chromosome Libraries.- A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method.- A Microtiter-Plate-Based High Throughput PCR Product Purification Method.