Biomolecular Crystallography

Bernhard Rupp is the CEO and founder of q.e.d. life science discoveries in California. Dr. Rupp established the protein drug target crystallography group at the University of California Lawrence Livermore National Laboratory in 1993 and expanded it into high throughput crystallization and structural genomics with focus on drug target structures and structure guided drug discovery. He was also a founding member of TB Structural Genomics Consortium. Dr. Rupp is a consultant for several US biotech ventures, universities, and instrument manufacturers. He is a member of the U.S. National Academy of Sciences Committee for Crystallography and NAS Keck Futures Initiatives panel. Dr. Rupp lectures regularly at NSF sponsored workshops on Biomolecular Crystallography and at the EMBO PEPC workshops on protein expression,

PART I: FROM SEQUENCE TO CRYSTALS
1. Introduction: Preparing Your Study
2. Protein Structure
3. Protein Crystallization
4. Proteins for Crystallography

PART II: FUNDAMENTALS OF PROTEIN CRYSTALLOGRAPHY
5. Crystal Geometry
6. Diffraction Basics
7. Statistics and Probability in Crystallography

PART III: FROM CRYSTALS TO DATA
8. Instrumentation and Data Collection

PART IV: DETERMINING YOUR STRUCTURE
9. Reconstruction of Electron Density and the Phase Problem
10. Experimental Phasing
11. Non-Crystallographic Symmetry and Molecular Replacement
12. Model Building and Refinement

PART V: MAKING SENSE OF YOUR STRUCTURE
13. Structure Validation, Analysis, and Presentation