Mitotic Exit

A Transgenic Method to Measure Mitotic Exit in Drosophila Embryos.- Generation of Cdc20 RNAi-Sensitive Cell Lines to Study Mitotic Exit.- Overexpression of GFP Fusions of Regulators of the SAC from Arabidopsis thaliana.- Production of SAC Proteins in a Baculovirus-Based Heterologous Gene Expression System.- Quantitative Live-Cell Imaging to Study Chromatin Segregation and Nuclear Reformation.- Determination of Anaphase Duration by Time-Lapse Microscopy in Budding Yeast.- Visualizing Cartwheel Disassembly Process During Mitosis in Fixed and Live Cells by Fluorescence Microscope.- FRET-FLIM for the Study of Protein-Protein Interactions Underpinning Mitosis Checkpoints.- Using FRET to Define Cdk1-Dependent Ordering of Events during Exit from Second Meiotic M-Phase in Oocytes.- Time-Lapse Imaging to Analyze Cell Fate in Response to Antimitotics.- The Role of Polo-Like Kinase 1 (PLK1) O-GlcNAcylation in Mitosis.- Luciferase-Based Reporter Assay for the Assessment of Aurora A-Kinase Activity in Mitotic Cycle.- Methods for Detecting Phosphorylated Proteins and Observing Intracellular Localization during Mitosis.- Studying Mitotic Phosphorylation in Drosophila.- Inducing Exit from Mitosis by Inactivating Cdk1/Cyclin B in Metaphase-Arrested Cells: A Tool to Study Mitotic Exit and Reestablishment of Interphase.- Targeting Mitotic Exit in Malignant Cells.- The Use of APC/C Antagonists to Promote Mitotic Catastrophe in Cancer Cells.