Epigenomics

DNA Methylation Analysis Using Bisulfite Pyrosequencing.- Post-Bisulfite Adaptor Tagging Based on an ssDNA Ligation Technique (tPBAT).- Reduced Representation Bisulfite Sequencing (RRBS).- Chromatin Immunoprecipitation Sequencing (ChIP-seq) for Detecting Histone Modifications and Modifiers.- ATAC-Seq Analysis of Accessible Chromatin: From Experimental Steps to Data Analysis.- Low-Input CUT&RUN for Mouse Oocytes and Preimplantation Embryos.- Imaging Chromatin Accessibility by Assay of Transposase-Accessible Chromatin with Visualization.- STREAMING-Tag System: Technology to Enable Visualization of Transcriptional Activity and Subnuclear Localization of Specific Endogenous Genes.- Bioinformatics Pipelines for Identification of Super-Enhancers and 3D Chromatin Contacts.- A Method for Detection of Somatic LINE-1 Insertions at the Single Cell Level from Postmortem Human Brain.- Solubilization of Mouse Sperm Chromatin for Sequencing Analyses Using a Chaperon Protein.- Efficient Targeted DNA Methylation with dCas9-Coupled DNMT3A-DNMT3L Methyltransferase.- Regulation of Gene Expression Using dCas9-SunTag Platforms.- Concatenated Coiled-Coil Tag for Highly Efficient, Small Molecule-Inducible Upregulation of Endogenous Mammalian Genes.- Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells.- A Split CRISPR–Cpf1 Platform for Inducible Gene Activation.- Targeted DNA Methylation in Mouse Early Embryos.- Generation of Epigenetic Disease Model Mice by Targeted Demethylation of the Epigenome.- In Vivo Tissue-Specific DNA Demethylation in Mouse Liver through a Hydrodynamic Tail Vein Injection.- Targeted Manipulation of Histone Modification in Medaka Embryos.