Glycan Microarrays
Springer-Verlag New York Inc.
978-1-0716-2150-9 (ISBN)
Authoritative and cutting-edge, Glycan Microarrays: Methods and Protocols aims to highlight the interdisciplinary nature of the development and use of the glycan microarray platform.
Neoglycoprotein and glycoprotein printing on a hydrogel functionalized microarray surface and incubation with labeled lectins.- Evaluation of glycan-binding specificity by glycoconjugate microarray with an evanescent-field fluorescence detection system.- Multiplex glycan bead array (MGBA) for high throughput and high content analyses of glycan binding proteins including natural anti-glycan antibodies.- Nanocube-based fluidic glycan array.- General strategies for glycan microarray data processing and analysis.- Calculating half maximal inhibitory concentration (IC50) values from glycomics microarray data using GraphPad Prism.- Synthetic plant glycan microarrays as tools for plant biology.- Mucin purification and printing natural mucin microarrays.- Bacterial microarrays for examining bacterial glycosignatures and recognition by host lectins.- Tissue glycome mapping: Lectin microarray-based differential glycomic analysis of formalin-fixed paraffin-embedded tissue sections.- Detection of autoantibodies using combinatorial glycolipid microarrays.- Glycan microarrays containing synthetic Streptococcus pneumoniae CPS fragments and their application to vaccine development.- Lectin-based protein microarray for the glycan analysis of colorectal cancer biomarkers: the insulin-like growth factor system.- Bacterial staining and profiling for glycan interactions on glycan microarrays for t-test calculation.- Preparation and fluorescent labelling of cell-derived micelles and profiling on glycan microarrays.
Erscheinungsdatum | 29.12.2022 |
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Reihe/Serie | Methods in Molecular Biology ; 2460 |
Zusatzinfo | 65 Illustrations, color; 7 Illustrations, black and white; XI, 253 p. 72 illus., 65 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Naturwissenschaften ► Biologie ► Biochemie |
Schlagworte | Cancer glycobiology • glycoprotein • microarray scanning • Mirage • neoglycoconjugate printing |
ISBN-10 | 1-0716-2150-5 / 1071621505 |
ISBN-13 | 978-1-0716-2150-9 / 9781071621509 |
Zustand | Neuware |
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