The Ubiquitin Code

Thioester and oxyester linkages in the ubiquitin system.- Getting to the root of branched ubiquitin chains: A review of current methods and functions.- Chemical synthesis of non-hydrolysable Ubiquitin(-like) hybrid chains.- Total Linear Chemical Synthesis of LC3A and LC3B.- A microbead-based flow cytometry assay to assess the activity of Ubiquitin and Ubiquitin-like conjugating enzymes.- Monitoring HECT ubiquitination activity in vitro.- RNAi-based screening for the identification of specific substrate-deubiquitinase pairs.- Strategies for monitoring ‘ubiquitin C-terminal hydrolase 1’ (Yuh1) activity.- Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome using Chain-Specific Nanobodies.- A mass spectrometry-based strategy for mapping modification sites for the Ubiquitin-like modifier NEDD8.- Isolation of the hepatic ubiquitome/NEDDylome by streptavidin pull-down assay in the biotinylated ubiquitin (bioUb)/biotinylated NEDD8 (bioNEDD8) transgenic mice.- The Lambda display technology: a useful tool for the identification of ubiquitin and ubiquitin like-binding domains.- SUMO-ID: a strategy for the identification of SUMO-dependent proximal interactors.- Analysis of ATG8 family members using LC3-Interacting Regions (LIR)-based molecular traps.- A computational tool for analysis of mass spectrometry data of ubiquitin enriched samples.- In-plate and in-gel assays for the assessment of proteasome activity in Caenorhabditis elegans.- Isolation of Proteasome-Trapped Peptides (PTPs) for degradome analysis.