Mitochondria

Fast Determination of Mitochondrial Metabolism and Respiratory Complex Activity in Permeabilized and Intact Cells.- Unbiased Millivolts Assay of Mitochondrial Membrane Potential in Intact Cells.- Rotenone Decreases Ischemia-induced Injury by Inhibiting Mitochondrial Permeability Transition – A study in Brain.- Assessment of Mitochondrial Complex II and III Activity In Brain Sections: a Histoenzymological Technique.- Measurement of mitochondrial (dys)function in cellular systems using Electron Paramagnetic Resonance (EPR): oxygen consumption rate and superoxide production.- Mitochondrial Calcium Handling in Isolated Mitochondria from a Guinea Pig Heart.- Characterizing the Electron Transport Chain---Structural Approach.- Characterizing the electron transport chain--functional approach using Extracellular Flux Analyzer on mouse tissue samples.- Simultaneous Acquisition of Mitochondrial Calcium Retention Capacity and Swelling to Measure Permeability Transition Sensitivity.- Measuring mitochondrial Function: From organelle to organism.- Mitochondrial Toxicity of Organic Arsenicals.- Measurements of Mitochondrial Respiration in Intact Cells, Permeabilized Cells, And Isolated Tissue Mitochondria using the Seahorse XF Analyzer.- Monitoring mitochondrial morphology and respiration in Doxorubicin-induced cardiomyopathy.- Creation of yeast models for evaluating the pathogenicity of mutations in the human mitochondrial gene MT-ATP6 and discovering therapeutic molecules.- In vivo analysis of mitochondrial protein synthesis in Saccharomyces cerevisiae mitochondrial tRNA mutants.- Visualizing mitochondrial importability of a protein using the yeast Bi-genomic Mitochondrial-Split-GFP strain and an ordinary fluorescence microscope.- Analysis of Mitochondrial Performance in Lymphocytes using Fluorescent Life--Time Imaging Microscopy.- Monitoring mitochondrial perturbationsduring infection.- Measuring the Mitochondrial Ubiquinone (Q) Pool Redox State In Isolated Respiring Mitochondria.- Experimental Setup For Investigation Of Acute Mitochondrial Oxygen Sensing In Primary Cells.- Assessing the redox status of mitochondria through the NADH/FAD+ ratio in intact cells.- Monitoring mitochondrial membrane potential in live cells using time-lapse fluorescence imaging.- Investigating mitochondrial Ca2+ dynamics in isolated mitochondria and intact cells: Application of fluorescent dyes and genetic reporters.- A plate reader-based measurement of the cellular ROS production using Dihydroethidium and MitoSOX.- Analysis of organisation and activity of mitochondrial respiratory chain complexes in primary fibroblasts using blue native PAGE.- Multiplexing Seahorse XFe24 and ImageXpress® Nano platforms for comprehensive evaluation of mitochondrial bioenergetic profile and neuronal morphology.- Computational Modelling of Mitochondria to Understand the Dynamics of Oxidative Stress.