Basophils and Mast Cells
Springer-Verlag New York Inc.
978-1-0716-0698-8 (ISBN)
Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease.- Purification of Basophils from Peripheral Human Blood.- Mast Cell Purification Protocols.- Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells.- Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs).- Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34+ Progenitor Cells.- Generation of Mast Cells from Murine Stem Cell Progenitors.- Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model.- The Absolute Basophil Count.- Mast Cell and Basophils Cell Lines: A Compendiu.- Use of Humanized RBL Reporter Systems for Detection of Allergen-specific IgE Sensitization in Human Serum.- Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for Detection of Allergen-specific IgE in Patient Sera Using Allergen Microarrays.- NPY-mRFP Rat Basophil Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation.- Use of Engineered Nanoparticles (ENPs) for the Study of High-affinity IgE Receptor FcεRI Receptor Engagement and Rat Basophil Leukemia (RBL) Cell degranulation.- Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques.- Basophil Activation Experiments in Immediate Drug Hypersensitivity: More Than a Diagnostic Aid.- Basophil Activation Techniques: Staining of Exteriorized Granule Matrix.- Measurement and Functional Analysis of the Mas-related G protein-coupled receptor MRGPRX2 on Human Mast Cells and Basophils.- The Mast Cell Activation Test (MAT).- Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique.- Measuring Histamine and Cytokine Release from Basophils and Mast Cells.- Detection of Mast Cells and Basophils by Immunohistochemistry.- Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry.- Mast Cell Migration and Chemotaxis Assayed by Microscopy.- Basophil Stimulation and Signaling Pathway.- Gene Silencing Approaches in Mast Cells and Primary Human Basophils.- Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells.- Mast Cell Mediated Reactions in vivo.- Identification of Murine Basophils by Flow Cytometry and Histology.
Erscheinungsdatum | 16.08.2021 |
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Reihe/Serie | Methods in Molecular Biology ; 2163 |
Zusatzinfo | 48 Illustrations, color; 2 Illustrations, black and white; XVIII, 380 p. 50 illus., 48 illus. in color. |
Verlagsort | New York, NY |
Sprache | englisch |
Maße | 178 x 254 mm |
Themenwelt | Studium ► Querschnittsbereiche ► Infektiologie / Immunologie |
Naturwissenschaften ► Biologie ► Zellbiologie | |
Schlagworte | Cell Culture • Knockout models • nanomaterials • Purification • signaling pathways |
ISBN-10 | 1-0716-0698-0 / 1071606980 |
ISBN-13 | 978-1-0716-0698-8 / 9781071606988 |
Zustand | Neuware |
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