Sequence space and properties of XPO1-dependent nuclear export signals - Oleh Rymarenko

Sequence space and properties of XPO1-dependent nuclear export signals

(Autor)

Buch | Softcover
166 Seiten
2021
Shaker (Verlag)
978-3-8440-7909-8 (ISBN)
48,80 inkl. MwSt
Exportin 1 (XPO1) mediates one of the most versatile protein transport pathways in the cell, exporting hundreds of different proteins from the nucleus to the cytoplasm. In this dissertation, I employ filamentous phage display coupled with high-throuput DNA sequencing to establish the sequence space of the most common type the nuclear export signals (NESes) recognized by XPO1. I confirm the role of the discovered sequence features in determining the nuclear export efficiency in the cell. I show the utility of the obtained information in engineering of NES-like sequences, including creating artificial NESes or NES-like sequences that bind XPO1 with high affinity. Additionally, I explore the use of the established sequence space parameters for prediction of NESes based on a protein sequence. This thesis could be of interest to biochemists and cell biologists interested in protein localization in the cell as well as specialists in protein-peptide interactions.
Erscheinungsdatum
Reihe/Serie Berichte aus der Biochemie
Verlagsort Düren
Sprache englisch
Maße 148 x 210 mm
Gewicht 249 g
Themenwelt Naturwissenschaften Biologie Biochemie
Schlagworte Nuclear • nuclear export • nuclear transport
ISBN-10 3-8440-7909-2 / 3844079092
ISBN-13 978-3-8440-7909-8 / 9783844079098
Zustand Neuware
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