Cryopreservation and Freeze-Drying Protocols

Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues.- Principles of Ice-Free Cryopreservation by Vitrification.- The Principles of Freeze-Drying and Application of Analytical Technologies.- Mathematical Modeling and Optimization of Cryopreservation in Single Cells.- Mathematical Modeling of Protectant Transport in Tissues.- Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation.- Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics.- High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics.- Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization.- DSC Analysis of Thermophysical Properties for Biomaterials and Formulations.- Osmometric Measurements of Cryoprotective Agent Permeation into Tissues.- Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes.- Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research.- Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements.- Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm.- Cryopreservation of Avian Semen.- Cryopreservation of Mouse Sperm for Genome Banking.- Cryopreservation of Marine Invertebrates: From Sperm to Complex Larval Stages.- Aseptic Cryoprotectant-Free Vitrification of Human Spermatozoa by Direct Dropping into a Cooling Agent.- Cryopreservation of Mammalian Oocytes: Slow Cooling and Vitrification as Successful Methods for Cryogenic Storage.- Vitrification of Porcine Oocytes and Zygotes in Microdrops on a Solid Metal Surface or Liquid Nitrogen.- Cryopreservation and Transplantation of Laboratory Rodent Ovarian Tissue for Genome Banking and Biomedical Research.- Cryopreservation and Thawing of Human Ovarian Cortex Tissue Slices.- Vitrification: A Simple and Successful Method for Cryostorage of Human Blastocysts.- Vitrificationof Equine In Vivo-Derived Embryos, after Blastocoel Aspiration.- Frozen Blood Reserves.- Isolation, Cryopreservation, and Characterization of iPSC-Derived Megakaryocytes.- Chemically Defined, Clinical-Grade Cryopreservation of Human Adipose Stem Cells.- Chemically-Defined and Xeno-Free Cryopreservation of Human Induced Pluripotent Stem Cells.- Protocol for Cryopreservation of Endothelial Monolayers.- Vitrification of Heart Valve Tissues.- Cryopreservation of Algae.- Cryopreservation of Fern Spores and Pollen.- Cryopreservation of Plant Cell Lines Using Alginate Encapsulation.- Cryopreservation of Plant Shoot Tips of Potato, Mint, Garlic, and Shallot Using Plant Vitrification Solution 3.- Cryopreservation of Seeds and Seed Embryos in Orthodox, Intermediate, and Recalcitrant Seeded Species.- Freeze-Drying of Proteins.- Freeze-Drying of Lactic Acid Bacteria: A Stepwise Approach for Developing a Freeze-Drying Protocol Based on Physical Properties.- Preservation of Mammalian Sperm by Freeze-Drying.- Freeze-Drying of Decellularized Heart Valves for Off-the-Shelf Availability.