Amyloid Proteins

Preparation of Pure Populations of Amyloid-β Protein Oligomers of Defined Size.- Preparation of Well-Defined and Stable β-Barrel Pore-Forming Amyloid-β42 Oligomers.- Unveiling Brain Aβ Heterogeneity through Targeted Proteomic Analysis.- Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments.- Generation and Characterization of Stable α-Synuclein Oligomers.- In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity.- Preparation of Tau Oligomers after Protein Extraction from Bacteria and Brain Cortices.- Purification and Characterization of Low-n Tau Oligomers.- Preparation and Characterization of Tau Oligomer Strains.- Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein and Tau under Native Conditions.- Preparation of Amyloidogenic  Aggregates from EF-Hand β –parvalbumin and S100 Proteins.- Kinetic Analysis of Amyloid Formation.- Mapping Amyloid Regions in Gad m 1with Peptide Arrays.- Non-Invasive Structural Analysis of Intermediate Species during Fibrillation: An Application of Small-Angle X-Ray Scattering.- Analysis of Covalent Modifications of Amyloidogenic Proteins using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation.- Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using The Real-Time Quaking-Induced-Conversion.- Bacterial Amyloids.- Addressing Intracellular Amyloidosis in Bacteria with RepA-WH1, a Prion-Like Protein.- Study of Amyloids Using Yeast.- Neurotoxic Ca2+ Signaling Induced by Amyloid-β Oligomers in Aged Hippocampal Neurons In Vitro.- Preparation and Culturing of Human Primary Vascular Cells.- Live Imaging of Pathological Tau Protein and Tau Antibodies in a Human Neuron-Like Cellular Model.- Effects of Amyloid-β Peptide on the Biology of Human Neural Stem Cells.- Development of Mouse Monoclonal Antibodies against Human Amyloid Fibril Proteins for Diagnostic and Research Purposes.- Identification and Characterization of Amyloid-β Accumulation in Synaptic Mitochondria.- Biochemical Properties of Pathology-Related Tau Species in Tauopathy Brains: An Extraction Protocol for Tau Oligomers and Aggregates.- Tau Assembly into Filaments.- Quantitative Metabolomics in Alzheimer´s Disease: Technical Considerations for Improved Reproducibility.- Detecting Circulating MicroRNAs as Biomarkers in Alzheimer’s Disease.- Luminescent Conjugated Oligothiophene Probe Applications for Fluorescence Imaging of Pure Amyloid Fibrils and Protein Aggregates in Tissues.- Characterization of Amyloid-β Plaques and Autofluorescent Lipofuscin Aggregates in Alzheimer´s Disease Brain: A Confocal Microscopy Approach.- In Vivo Imaging of Tauopathy in Mice.- In Vivo Evaluation of Neuronal Transport in Murine Models of Neurodegeneration using Manganese-Enhanced MRI.