Fibrosis

Human Fibrotic Diseases: Current Challenges in Fibrosis Research.- The Bleomycin Model of Pulmonary Fibrosis.- Intra-Dermal Injections of Bleomycin to Model Skin Fibrosis.- Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology.- Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars.- Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis.- Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension.- Mouse Models of Corneal Scarring.- Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype after Ischemia.- Characterization of Mesenchymal-Fibroblast Cells using the Col1a2 Promoter/Enhancer.- Isolation and Culture of Primary Murine Hepatic Stellate Cells.- Isolation and Culture of Adipose-Derived Stromal Cells from Sub-Cutaneous Fat.- Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting.- Detection of Infiltrating Mast Cells using a Modified Toluidine Blue Staining.- Cell-Populated Collagen Lattice Models.- Traction Force Measurement using Deformable Microposts.- Mechanical Deformation of Cultured Cells with Hydrogels.- Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis.- Type I Collagen Purification from Rat Tail Tendons.- Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments.- Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis.- Collagen Quantification in Tissue Specimens.- Methods for the Assessment of Active Transforming Growth Factor-β in Cells and Tissues.- Visualizing in Vitro Type I Collagen Fibrillogenesis  by Transmission Electron Microscopy.- Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks.- Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections.- Probing Collagen Organization: Practical Guide for Second Harmonic Generation (SHG) Imaging.- Methods for Quantifying Fibrillar Collagen Alignment.- Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM.- Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens.- Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research.