Development of a Fully Integrated “Sample-In-Answer-Out” System for Automatic Genetic Analysis

For internal review: Dr. Bin Zhuang Supervisor: Prof. Jing Cheng, School of Medicine, Tsinghua University Current Affiliation: National Engineering Research Center for Beijing Biochip Technology, Capitalbio Corporation Research Area: Biomedical engineering, microfluidic, nucleotide analysis, electrophoresis, biochemistry Publications: [1] Gan, W.P.,# Zhuang, B.,# Zhang, P.F., Han, J.P., Li, C.X., and Liu, P. (2014). A filter paper-based microdevice for low-cost, rapid, and automated DNA extraction and amplification from diverse sample types. Lab on a Chip 14, 3719-3728. [2] Zhuang, B., #  Gan, W.P., Wang, #  S.Q., Han, J.P., Xiang, G.X., Li, C.X., Sun, J., Liu, P. (2015). A fully automated sample preparation microsystem for genetic testing of hereditary hearing loss using two-color multiplex allele-specific PCR. Analytical Chemistry, 87(2):1202-1209. [3] Zhuang, B., #  Han, J.P., #  Xiang, G.X., Gan, W.P., Wang, S.Q., Wang, D., Wang, L., Sun, J., Li, C.X., Liu, P. (2016). A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms. Lab on a Chip 16, 86-95.

Introduction.- The construction of a general platform for capillary electrophoresis.- Integrated module for automatic DNA extraction and amplification.- A fully-integrated genetic analysis system.- Conclusion and prospects for future work.