Cytokine Protocols

A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender Discovery.-The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA Levels.-Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVID.-Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues.- Evaluating Posttranscriptional Regulation of Cytokine Genes.-Cloning of Cytokine 3' Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay.-Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivo.-Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor.- Control of the Interferon Response in RNAi Experiments.-shRNA-induced Interferon-Stimulated Gene Analysis.-Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells.-Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins.-In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic Cells.-A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH Cells.