Notch Signaling

Introduction to Notch Signaling.- Genetic Screens to Identify New Notch Pathway Mutants in Drosophila.- Structure-function Analysis of Drosophila Notch using Genomic Rescue Transgenes.- Overview of Genetic Tools and Techniques to Study Notch Signaling in Mice.- Immunohistochemical Tools and Techniques to Visualize Notch in Drosophila melanogaster.- Antibody Uptake Assay and in vivo Imaging to Study Intracellular Trafficking of Notch and Delta in Drosophila.- Tracking Trafficking of Notch and its Ligands in Mammalian Cells.- Visualizing Notch Signaling in vivo in Drosophila Tissues.- Monitoring Notch Activity in the Mouse.- Notch Signaling Assays in Drosophila Cultured Cell Lines.- Monitoring Notch Activation in Cultured Mammalian Cells: Transcriptional Reporter Assays.- Monitoring Notch Activation in Cultured Mammalian Cells: Luciferase Complementation Imaging Assays.- Visualization of Notch Signaling Oscillation in Cells and Tissues.- Proteomic Analysis of the Notch Interactome.- Bacterial Expression and in vitro Refolding of Limited Fragments of the Notch Receptor and its Ligands.- Analyzing the Post-translational Modification Status of Notch using Mass Spectrometry.- Assay to Probe Proteolytic Processing of Notch by γ-secretase.- Analyzing the Nuclear Complexes of Notch Signaling by Electrophoretic Mobility Shift Assay.- Identifying Direct Notch Transcriptional Targets using the GSI-washout Assay.- Probing the Epigenetic Status at Notch Target Genes.- Notch-ligand Binding Assays in Drosophila Cells.- Modeling Notch Signaling: A Practical Tutorial.- Small Molecules that Inhibit Notch Signaling.- Application and Evaluation of Anti-Notch Antibodies to Modulate Notch Signaling.- Application of Anti-ligand Antibodies to Inhibit Notch Signaling.