Advanced Techniques in Diagnostic Microbiology

Yi-Wei Tang, Chief of Clinical Microbiology Service, Memorial Sloan-Kettering Cancer Center Charles W. Stratton, Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine

Automated blood culture systems.-  Bacteriology full automation.- Advanced phenotypic systems and instruments for microbial identification.- Phenotypic antimicrobial resistance techniques.- Rapid microbial antigen test.- Advanced Antibody Detection.- Host Response-Based Biomarkers.- Functional Assessment of Microbial Infections by Real Time Cell Analysis System.- Cellular Fatty Acid-Based Microbial Identification and Antimicrobial Susceptibility Testing.- MALDI-TOF Mass Spectrometry.- Transcriptomic Techniques in Diagnostic Microbiology.- Metabolic Techniques in Diagnostic Microbiology.- Nucleic Acid Extraction and Enrichment.- Non-Amplified Probe-Based Microbial Detection and Identification.- Molecular Typing Techniques.- PCR and Derivatives.- Non-PCR Target Amplification Techniques.- Probe Amplification Techniques, LCR, CPT, REM.- Signal amplification techniques, bDNA, Invader, Digene.- Real-time and digital PCR for nucleic acid quantification.- Direct sequencingfor amplification product identification.- Solid and suspension arrays amplification product detection and identification.- Amplification product real-time identification through FRET.- PCR Mass Spectrometry.- Nucleic acid amplicons detected and identified by magnetic resonance.- Techniques and methods for carryover contamination control.