Type-1 Diabetes

Methods and Protocols

Kathleen M. Gillespie (Herausgeber)

Buch | Hardcover
213 Seiten
2016 | 1st ed. 2016
Humana Press Inc. (Verlag)
978-1-4939-3641-0 (ISBN)

Lese- und Medienproben

Type-1 Diabetes -
106,99 inkl. MwSt
The aim of this volume is to present current methodologies to predict and understand the pathogenesis of Type-1 Diabetes for clinical and non-clinical researchers.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.



Practical and reliable, Type-1 Diabetes: Methods and Protocols will aid researchers in using these methods to advance their own studies.

Type 1 Diabetes: Prediction And Prevention: Current Perspectives.- The HLA.- Pancreatic Beta Cell Survival and Signaling Pathways: Effects of Type 1 Diabetes-Associated Genetic Variants.- Islet Autoantibody Analysis: Radioimmunoassays.- Islet Autoantibody Analysis: Non-Radioactive Assays - Electrochemiluminescence.- Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function in Type 1 Diabetes.- Histology of the Type 1 Diabetes Pancreas.- Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model of Type 1 Diabetes.- Tracking Immunological Responses of Islet Antigen-Specific T Cells In The Non Obese Diabetic (NOD) Mouse Model Of Type 1 Diabetes.- Adoptive Transfer of Autoimmune Diabetes using Immunodeficient Non Obese Diabetic (NOD) Mice.- Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes.- FISH with Concomitant Immunofluorescence.- Laser Capture Microdissection:  Single Pancreatic Cell DNA Profiling.- The Gut Microbiome in the NOD Mouse.- Molecular Methods and Protein Synthesis for Definition of Autoantibody Epitopes. 








 

Erscheinungsdatum
Reihe/Serie Methods in Molecular Biology ; 1433
Zusatzinfo 33 Illustrations, color; 11 Illustrations, black and white; X, 213 p. 44 illus., 33 illus. in color.
Verlagsort Totowa, NJ
Sprache englisch
Maße 178 x 254 mm
Themenwelt Medizinische Fachgebiete Innere Medizin Diabetologie
Medizin / Pharmazie Studium
Naturwissenschaften Biologie Genetik / Molekularbiologie
Schlagworte Diabetes • Methodologies • pathogenesis • therapies • Type 1 Diabetes
ISBN-10 1-4939-3641-7 / 1493936417
ISBN-13 978-1-4939-3641-0 / 9781493936410
Zustand Neuware
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