Post-Transcriptional Gene Regulation

Introduction to Bioinformatics Resources for Post-Transcriptional Regulation of Gene Expression.- A Computational Approach for the Discovery of Protein-RNA Networks.- Transcriptional Regulation with CRISPR/Cas9 Effectors in Mammalian Cells.- Studying the Translatome with Polysome Profiling.- Exploring Ribosome Positioning on Translating Transcripts with Ribosome Profiling.- Studying Isoform-Specific Recruitment to Polyribosomes with Frac-Seq.- Use of the Pbutr Reporter System for Scalable Analysis of 3' UTR-Mediated Gene Regulation.- Comprehensive Identification of RNA-Binding Proteins by RNA Interactome Capture.- Identifying RBP Targets with RIP-Seq.- PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites.- Profiling the Binding Sites of RNA-Binding Proteins with Nucleotide Resolution Using Iclip.- A Pipeline for PAR-CLIP Data Analysis.- Capture and Identification Of Mirna Targets By Biotin Pulldown And RNA-Seq.- Identification of Mirna-Target RNA Interactions Using CLASH.- Genome-Wide Analysis of A-To-I RNA Editing.- Nucleotide-Level Profiling Of M5c RNA Methylation.- Probing N6-Methyladenosine (M6a) RNA Modification in Total RNA with SCARLET.- Genome-Wide Identification of Alternative Polyadenylation Events Using 3’T-Fill.- Genome-Wide Profiling Of Alternative Translation Initiation Sites.- Genome-Wide Study Of Mrna Isoform Half-Lives.- Visualizing Mrna Dynamics in Live Neurons and Brain Tissues.- Single Molecule Live-Cell Visualization of Pre-Mrna Splicing.