Gene Knockout Protocols
Humana Press Inc. (Verlag)
978-1-62703-834-8 (ISBN)
Gene Modification in ES Cells.- Overview on Mouse Mutagenesis.- Construction of Gene-Targeting Vectors by Recombineering.- Gene-Trap Vectors and Mutagenesis.- Chromosome Engineering in ES Cells.- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides.- Generation of shRNA Transgenic Mice.- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate.- Stem Cell Manipulation.- Gene Targeting in Mouse Embryonic Stem Cells.- Manipulating Mouse Embryonic Stem Cells.- ES Cell Line Establishment.- Generation of Double-Knockout Embryonic Stem Cells.- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro.- Cloning of ES Cells and Mice by Nuclear Transfer.- Genetically Engineered Mice.- Isolation, Microinjection and Transfer of Mouse Blastocysts.- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos.- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos.- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes.- Inducible Cre Mice.- Creation and Use of a Cre Recombinase Transgenic Database.- Transposon Mutagenesis in Mice.- Lentiviral Transgenesis.- Sperm Cryopreservation and In Vitro Fertilization.- Phenotype Analysis.- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes.- Pathologic Phenotyping of Mutant Mice.- Systemic First-Line Phenotyping.
Erscheint lt. Verlag | 30.10.2014 |
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Reihe/Serie | Methods in Molecular Biology ; 530 |
Zusatzinfo | XVI, 516 p. |
Verlagsort | Totowa, NJ |
Sprache | englisch |
Maße | 193 x 260 mm |
Themenwelt | Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik |
Naturwissenschaften ► Biologie ► Biochemie | |
Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
Naturwissenschaften ► Biologie ► Zoologie | |
Schlagworte | chromosome • Cryopreservation • DNA • ES cells • Gene modification • genes • Genetically engineered mice • Genetics • Molecular Biology • Mouse genome • Mutagenesis techniques • Mutant • Phenotype analysis • Stem cell manipulation |
ISBN-10 | 1-62703-834-5 / 1627038345 |
ISBN-13 | 978-1-62703-834-8 / 9781627038348 |
Zustand | Neuware |
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