Rhodopsin

Beata Jastrzebska, Ph.D. Department of Pharmacology, W317 Case Western Reserve University 10900 Euclid Avenue Cleveland, OH 44106-4965

The G Protein-Coupled Receptor Rhodopsin: A Historical Perspective.- Rhodopsin Purification from Dark Adapted Bovine Retina.- Mammalian Expression, Purification, and Crystallization of Rhodopsin Variants.- Imaging of Rhodopsin Crystals with Two-Photon Microscopy.- Functional Stability of Rhodopsin in a Bicelle System: Evaluating G-Protein Activation by Rhodopsin in Bicelles.- The Rhodopsin-Arrestin-1 Interaction in Bicelles.- Detection of Structural Waters and Their Role in Structural Dynamics of Rhodopsin Activation.- Probing Conformational Changes in Rhodopsin Using Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry.- Analysis of Conformational Changes in Rhodopsin by Histidine Hydrogen-Deuterium Exchange.- Investigation of Rhodopsin Dynamics in its Signaling State by Solid-State Deuterium NMR Spectroscopy.- Sequential Structural Changes in Rhodopsin Occurring upon Photoactivation.- Dynamic Single-Molecule Force Spectroscopy of Rhodopsin in Native Membranes.- High-Resolution Atomic Force Microscopy Imaging of Rhodopsin in Rod Outer Segment Disk Membranes.- Detection of Rhodopsin Dimerization in situ by PIE-FCCS, a Time-Resolved Fluorescence Spectroscopy.- Oligomeric State of Rhodopsin within Rhodopsin-Transducin Complex Probed with Succinylated Concanavalin A.- Quantification of Arrestin-Rhodopsin Binding Stoichiometry.- Rhodopsin Transient Complexes Investigated by Systems Biology Approaches.- Three Dimensional Architecture of Murine Rod Cilium Revealed by Cryo-EM.- Monitoring of Rhodopsin Trafficking and Mistrafficking in Live Photoreceptors.- Measurements of Rhodopsin Diffusion within Signaling Membrane Microcompartments in Live Photoreceptors.- Kinetics of Rhodopsin’s Chromophore Monitored in a Single Photoreceptor.- Supplementation with Vitamin A Derivatives to RescueVision in Animal Models of Degenerative Retinal Diseases.- Sustained Delivery of Retinoids to Prevent Photoreceptor Death.- High-Throughput Screening Assays to Identify Small-Molecules Preventing Photoreceptor Degeneration Caused by the Rhodopsin P23H Mutation.- Gene Therapy to Rescue Retinal Degeneration Caused by Mutations in Rhodopsin.