Physiology and Pathophysiology of Plasma Protein Metabolism (eBook)

Front Cover 1
Physiology and Pathophysiology of Plasma Protein Metabolism 6
Copyright Page 7
Table of Contents 8
PREFACE 12
PART 1: SEPARATION, PURIFICATIONAND LABELLING OF PROTEINS 14
CHAPTER 1. CHEMICAL CHANGES DUE TO IODINATIONAND METABOLIC BEHAVIOUROF HUMAN SERUM ALBUMIN 16
INTRODUCTION 16
SELECTION OF THE ALBUMIN BATCH 16
EFFECT OF PROGRESSIVE IODINATION 17
DAMAGING EFFECT ASSOCIATED TO THE DIFFERENT LABELLING PROCEDURES 23
SUMMARY 25
REFERENCES 25
PART 2: KINETICS 30
CHAPTER 2. WHOLE-BODY COUNTING IN METABOLICSTUDIES OF 131 I-LABELLED PROTEINS 32
THE WHOLE-BODY COUNTER 32
METABOLIC STUDIES 34
THE CHANGING SELF-ABSORPTION 34
THE VALUE O F WHOLE-BODY COUNTING 36
SUMMARY 39
REFERENCES 39
CHAPTER 3. DECONVOLUTION TECHNIQUES FOR THEANALYSIS OF SHORT-TERM METABOLICSTUDIES WITH RADIOIOD1NATED ALBUMINf 42
PRINCIPLE OF THE METHOD 43
REFERENCES 47
CHAPTER 4. METABOLISM OF BENCE JONES PROTEIN 50
REFERENCES 54
PART 3: REGULATION AND SYNTHESIS OF PROTEIN 64
CHAPTER 5. SHORT - TERM MEASUREMENTS OF SERUM PROTEIN SYNTHESIS 66
SUMMARY 71
REFERENCES 71
CHAPTER 6. ALBUM IN SYNTHETIC IN LIVER DISEASE 74
SUMMARY 78
REFERENCES 80
CHAPTER 7. ANALBUMINAEMIA—A STUDY OF ALBUMINAND TRANSFERRIN METABOLISM 88
SUMMARY 96
ACKNOWLEDGEMENTS 96
REFERENCES 96
CHAPTER 8. SITES OF PROTEIN CATABOLISM 100
REFERENCE 104
CHAPTER 9. HORMONAL CONTROL OF PLASMAAL BUMIN SYNTHESIS 108
MATERIAL AND METHODS 108
RESULTS 109
REFERENCE 111
CHAPTER 10. KINETIC STUDIES OF IMMUNOGLOBULIN METABOLISM IN IMMUNOLOGIC DEFICIENCY 114
METHODS 114
SUMMARY 120
REFERENCES 120
CHAPTER 11. METABOLISM OF NORMAL y M - GLOBULIN IN NORMALS 122
MATERIAL AND METHODS 122
PROCEDURE 123
RESULTS 126
DISCUSSION 126
CONCLUSION 127
REFERENCES 127
CHAPTER 12. THE METABOLISM OF AUTOLOGOUS Ig M AND 19S RHEUMATOID FACTOR 128
METHODS 128
MATERIAL 130
RESULTS 131
REFERENCES 135
CHAPTER 13. THE TURNOVER OF COLD AGGLUTININAND ITS SPLIT PRODUCTS 138
METHODS 139
RESULTS AND DISCUSSION 140
REFERENCES 144
CHAPTER 14. METABOLISM OF ALBUMIN AND yG-GLOBULIN IN MAN DURING ALBUM ININFUSIONS AND DURING PLASMAPHERESIS 150
METHODS 151
DISCUSSION 156
REFERENCES 157
CHAPTER 15. THE METABOLISM OF LOW-DENSITY (ß)LIPOPROTEINS IN HEALTHAND DISEASE 160
I. CHARACTERISTICS AS SERUM PROTEIN 160
II. CHARACTERISTICS AS CARRIER PROTEIN 161
III. EXTRAVASCULAR DEPOSITION 162
REFERENCES 162
CHAPTER 16. PROTEIN METABOLISM IN OLD PEOPLE 164
MATERIAL 164
METHODS 164
RESULTS AND DISCUSSION 165
SUMMARY 166
REFERENCES 166
PART 4: PROTEIN-LOSING SYNDROME 168
CHAPTER 17. CONSTRICTIVE PERICARDITIS WITHPROTEIN-LOSING ENTEROPATHY.ROLE OF THE LYMPHATIC SYSTEM 170
CASE REPORTS 170
DISCUSSION 174
ACKNOWLEDGEMENTS 177
REFERENCES 177
CHAPTER 18. CLINICALLY USEFUL PARAMETERSOF SERUM ALBUMIN METABOLISMIN MAN 180
METHODS 181
MATERIAL 182
RESULTS 182
DISCUSSION 184
REFERENCES 186
CHAPTER 19. IMMUNOLOGICAL DISORDERS IN PATIENTSWITH INTESTINAL LYMPHANGIECTASIA 188
ANTIBODY STIMULATION STUDIES 190
EVALUATION OF CELLULAR HYPERSENSITIVITY 191
SUMMARY 192
REFERENCES 192
CHAPTER 20. IDIOPATHIC HYPOPROTEINAEMIA WITHOUT PROTEIN LOSS 196
CASE REPORT 196
RESULTS 197
DISCUSSION 199
SUMMARY 200
DISCUSSION 200
CHAPTER 21. THE GASTROINTESTINAL TRACTIN DYSGAMMAGLOBULINEMIAf 202
VILLOUS ATROPHY 204
NODULAR HYPERPLASIA 204
NORMAL JEJUNAL HISTOLOGY 204
CHRONIC INFLAMMATION—(?) GRANULOMA 208
DISCUSSION 208
SUMMARY 210
REFERENCES 210
CHAPTER 22. PATTERNS OF SERUM ALBUMIN METABOLISM IN RENAL DISEASESf 214
MATERIAL AND METHODS 215
PREPARATION OF SAMPLES AND COUNTING 217
CALCULATIONS 217
RESULTS 218
SUMMARY 228
REFERENCES 228
CHAPTER 23. PLASMA PROTEIN METABOLISMIN THE NEPHROTIC SYNDROME IN ADULTS 232
MATERIAL 232
METHODS 232
RESULTS 233
REFERENCES 238
CHAPTER 24. ALBUMIN DISTRIBUTIONIN THE NEPHROTIC RAT 240
METHODS 240
RESULTS 241
DISCUSSION 242
REFERENCES 243
PART 5: VARIA 248
CHAPTER 25. PLASMA CLEARANCE OF 57COBALT-LABELLED VITAMIN B12 BOUND IN VITRO AND IN VIVO TO TRANSCOBALAMIN 1 AND II 250
METHODS 250
RESULTS 251
CONCLUSION 252
REFERENCES 253
CHAPTER 26. INTRINSIC FACTOR AUTOANTIBODIES 254
MATERIALS AND METHODS 255
RESULTS 255
DISCUSSION 256
REFERENCES 256
CHAPTER 27. EFFECTS OF LOW-MOLECULAR WEIGHTDEXTRAN INFUSION ON PLASMA PROTEINDISTRIBUTION AND CATABOLISM 258
DISCUSSION 262
ACKNOWLEDGEMENTS 265
REFERENCES 265
CHAPTER 28. CHANGES IN THE COLLOID OSMOTICPRESSURE OF SERUM FROM PATIENTSWITH BURNS OR OTHER INJURIES 268
METHODS 268
RESULTS 270
DISCUSSION 275
SUMMARY 276
ACKNOWLEDGEMENTS 277
REFERENCES 277
CONCLUDING REMARKS 280