Epstein-Barr Virus Protocols

Genome and Transcript Analyses.- Epstein-Barr Virus.- Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR.- Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids.- Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs).- Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells.- Visualizing EBV Expression Patterns by FISH.- Viral Detection.- In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products.- Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections.- Detection of EBV Infection at the Single-Cell Level.- Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo.- Culture Methods.- Virus Isolation.- Generation of Lymphoblastoid Cell Lines (LCLs).- Cell Cycle Distribution of B-Lymphocytes and Cell Lines.- of Plasmid Vectors into Cells Via Electroporation.- Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene.- Transient Gene Expression and MACS Enrichment.- In Vitro Assays to Study Epithelial Cell Growth.- In Vitro Assays to Study Epithelial Cell Differentiation.- Cell Sensitivity Assays.- Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation.- Immune Assays.- Regression Assay.- Generation of Polyclonal EBV-Specific CTL Cultures and Clones.- Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs.- Limiting Dilution Assay.- Viral Protein Detection.- Antibodies for Detecting EBV Latent Proteins.- Detection of EBV Latent Proteins by Western Blotting.- Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis.- Protein-Protein, Protein-DNA, and Protein-RNA Interactions.- The Yeast Two-Hybrid Assay to Identify Interacting Proteins.- Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins.- Magnetic DNA Affinity Purification of a Cellular Transcription Factor.- Screening an Expression Library with a DNA Binding Site.- Analysis of DNA Binding Proteins by Mobility Shift Assay.- Analysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs.- Protein Activity Assays.- Chimeric and Mutated Variants of LMP1.- Assaying the Activity of Kinases Regulated by LMP1.- In Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase Activities.- Tissue and In Vivo Protocols.- Analysis of Apoptosis in Tissue Sections.- Considerations in Generating Transgenic Mice.- In Vivo Assay of Cellular Proliferation.- Topical Chemical Carcinogen Treatment in Mice.- Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture.- Selection and Enrichment of B Cells from Lymphoid Tissues.- Detection of Immunoglobulin Gene Rearrangements.