Exocytosis and Endocytosis

Pharmacological Inhibitors of Exocytosis and Endocytosis:  Novel Bullets for Old Targets.- Systematic Analysis of Endocytosis by Cellular Perturbations.- Real Time Detection of SNARE Complexes with FRET Using the Tetracysteine System.- Profiling Lysine Ubiquitination by Selective Enrichment of Ubiquitin Remnant-Containing Peptides.- Secretion of Circular Proteins Using Sortase.- Fractionation of Subcellular Membrane Vesicles of Epithelial and Non-Epithelial Cells by OptiPrepTM Density Gradient Ultracentrifugation.- Combining Pulsed SILAC Labeling and Click-Chemistry for Quantitative Secretome Analysis.- Probabilistic Density Maps to Study the Spatial Organization of Endocytosis.- Use of Kaede and Kikume Green-Red Fusions for Live Cell Imaging of G Protein-Coupled Receptors.- Halo Tag as a Tool to Investigate Peroxisome Dynamics in Cultured Mammalian Cells.- SNAP-Tag to Monitor Trafficking of  Membrane Proteins in Polarized Epithelial Cells.- FLAsH-PALM:  Super-Resolution Pointillist Imaging with FLAsH-tetracysteine Labeling.- Analysis of Protein Dynamics with Tandem Fluorescent Protein Timers.- Synchronization of Secretory Cargo Trafficking in Populations of Cells.- Use of Transmembrane FRET to Investigate the Internalization of Glycosylated Proteins.- A Method to Rapidly Induce Organelle-specific Molecular Activities and Membrane Tethering.- A Novel Pair of Split Venus Fragments to Detect Protein-Protein Interactions by In Vitro and In Vivo Biomolecular Fluorescence Complementation Assays.- Real Time Investigation of Plasma Membrane Deformation and Fusion Pore Expansion Using Polarized Total Internal Reflection Fluorescence Microscopy.- Nanocones to Study Initial Steps of Endocytosis.- A Novel Permeabilization Protocol to Obtain Intracellular 3D Immunolabeling for Electron Tomography.- VIS2FIX: Rapid Chemical Fixation of Vitreous Sections for Immuno-Electron Microscopy.- Chemical Genomics: Characterizing Target Pathways for Bioactive Compounds Using theEndomembrane Trafficking Network.- Application of RNAi Technology and Fluorescent Protein Markers to Study Membrane Traffic in C. elegans.- Visualization of Clathrin-Mediated Endocytosis in Live Drosophila Egg Chambers.- Novel Extraction Protocol to Probe the Role of Cholesterol in Synaptic Vesicle Recycling.- Microfluidic Devices for Imaging Trafficking Events In Vivo Using Genetic Model Organisms.- The “In Situ” Proximity Ligation Assay to Probe Protein-Protein Interactions in Intact Tissues.- Probing the Role of the Actin Cytoskeleton During Regulated Exocytosis by Intravital Microscopy.- Measurement of Dynamic F-actin Changes During Exocytosis.