Neutrophil Methods and Protocols

The Role of Neutrophils in the Immune System: An Overview.- Isolation of Human Neutrophils.- Neutrophil Isolation from Non-Human Species.- Collection of In vivo Transmigrated Neutrophils from Human Skin.- Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular Markers.- Rho Family and Rap GTPase Activation Assays.- Measurement of Phospholipid Metabolism in Intact Neutrophils.- Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium Signals in Neutrophils.- Analysis of Electrophysiological Properties and Responses of Neutrophils.- Analysis of Neutrophil Apoptosis.- Microinjection Methods for Neutrophils.- Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells.- Neutrophil Migration Through Extracellular Matrix.- Spinning Disk Confocal Imaging of Neutrophil Migration in Zebrafish.- Detection of Bi-Directional Signalilng During Integrin Activation and In Neutrophil Adhesion.- Immunofluorescence and Confocal Microscopy of Neutrophils.- Expression of Genetically-Encoded Fluorescent Probes to Monitor Phospholipid Dynamics in Live Neutrophils.- Quantitative Assessment of Neutrophil Phagocytosis Using Flow Cytometry.- Analysis of Neutrophil Bactericidal Activity.- Induction and Quantification of Neutrophil Extracellular Traps.- Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes.- Cell-free NADPH Oxidase Assays: “In vitro Veritas”.- Assessment of Priming of the Human Neutrophil Respiratory Burst.- Affinity Purification and Reconstitution of Human Phagocyte Flavocytochrome b for Detection of Conformational Dynamics in the Membrane.- Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies.- Genome-Scale Transcript Analyses with Human Neutrophils.- Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils.- High Purity Neutrophil Isolation from Human Peripheral Blood and Saliva for Transcriptome Analysis.- Detection of Intact Transcription Factors in Human Neutrophils.- Disorders of Neutrophil Function: An Overview.- Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders.- Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency.