Membrane Potential Imaging in the Nervous System (eBook)

Dejan Zecevic (b. Belgrade 1948) is a Research Scientist at the Department of Cellular and Molecular Physiology, Yale University School of Medicine. He received the PhD in Biophysics from The University of Belgrade, Serbia and was trained in the laboratory of Dr Lawrence Cohen who initiated the field of voltage-sensitive dye recording. Dejan is the pioneer of intracellular voltage-sensitive dye imaging technique, a unique and a cutting edge technology for monitoring the membrane potential fluctuation in dendritic spines and fine branches. Marco (b. Milan 1970) is first class INSERM researcher (CR1) working at the Grenoble Institute of Neuroscience. He graduated in physics at the University of Genoa and received his PhD in biophysics from the International School for Advanced Studies in Trieste. He worked at the National Institute for Medical Research in London, at Yale University and at the University of Basel. Marco is expert on several optical techniques applied to neurophysiology. Marco and Dejan collaborated for a number of years using voltage-imaging and calcium imaging approaches to study mechanisms underlying synaptic plasticity.

Chapter 1: Historical overview and general methods of membrane potential imaging
Lawrence B Cohen

Chapter 2: Design and use of organic voltage sensitive dyes
Leslie M Loew

Chapter 3: Imaging submillisecond membrane potential changes from individual regions of single axons, dendrites and spines
Marco Canepari, Marko Popovic, Kaspar Vogt, Knut Holthoff, Arthur Konnerth, Brian M Salzberg, Amiram Grinvald, Srdjan D Antic and Dejan Zecevic

Chapter 4: Combined voltage and calcium imaging and signal calibration
Marco Canepari, Peter Saggau and Dejan Zecevic

Chapter 5: Use of fast-responding voltage-sensitive dyes for large-scale recording of neuronal spiking activity with single-cell resolution
William N Frost, Jean Wang, Christopher J Brandon, Caroline Moore-Kochlacs, Terrence J Sejnowski and Evan S Hill

Chapter 6: Monitoring integrated activity of individual neurons using FRET-based voltage-sensitive dyes
Kevin L Briggman, William B Kristan, Jesús E González, David Kleinfeld and Roger Y Tsien

Chapter 7: Monitoring population membrane potential signals from neocortex
Xiaoying Huang, Weifeng Xu, Kentaroh Takagaki and Jian-young Wu

Chapter 8: Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryos
Yoko Momose-Sato, Katsushige Sato and Kohtaro Kamino

Chapter 9: Imaging the dynamics of mammalian neocortical population activity in-vivo
Amiram Grinvald, David Omer, Shmuel Naaman and Dahlia Sharon

Chapter 10: Imaging the dynamics of neocortical population activity in behaving and freely moving mammals
Amiram Grinvald and Carl CH Petersen

Chapter 11: Monitoring membrane voltage using two-photon excitation of fluorescent voltage-sensitive dyes
Jonathan AN Fisher and Brian M Salzberg

Chapter 12: Random-access multiphoton microscopy for fast three-dimensional Imaging
Gaddum Duemani Reddy and Peter Saggau

Chapter 13: Second harmonic imaging of membrane potential
Leslie M. Loew and Aaron Lewis

Chapter 14: Genetically encoded protein sensors of membrane potential
Lei Jin, Hiroki Mutoh, Thomas Knopfel, Lawrence B Cohen, Thom Hughes, Vincent A Pieribone, Ehud Y Isacoff, Brian M Salzberg, and Bradley J Baker