Practical Neutron Protein Crystallography

Marc-Michael Blum is an independently working specialist for applying biotechnological methods based in Munich, Germany. He obtained his Ph.D. in Biophysical Chemistry in the group of Heinz Ruterjans at Frankfurt University, where he used both X-ray and neutron diffraction methods to study enzyme structure and mechanism. Paul Langan leads the Proteomics and Neutron Protein Crystallography team at the Los Alamos National Laboratory (USA) and directs the neutron protein crystallography facility at the Los Alamos Neutron Scattering Center. After obtaining a Ph.D. in biophysics from Keele University (UK), he worked at Oxford University and Grenoble, developing crystallographic methods for the study of biomolecules. He is also a past chairman of the American Crystallographic Associations's neutron scattering group, and a member of the neutron scattering commission of IUPAC.

INTRODUCTION Historical Introduction Scope of Neutron Protein Crystallography Complementarity to other Techniques Advantages and Disadvantages Future Trends Planning a Neutron Study SAMPLE PREPARATION Planning Sample Preparation Cloning, Expression and Perdeuteration Crystallization and Solvent Exchange Room Temperature and Cryo Sample Techniques DATA COLLECTION Planning Data Collection Monochromatic Techniques Quasi-Laue Techniques Time-of-Flight Techniques DATA REDUCTION Monochromatic Data Quasi-Laue Techniques Time-of-Flight Techniques STRUCTURE DETERMINATION AND ANALYSIS Planning Structure Refinement PHENIX nCNS Shelx HDX Analyses Water Building Determining Protonation States Hydrogen Bond Analyses Depositing Structures WALK-THROUGH EXAMPLES PCS: DFPase ADI D19: XI BIX BioDiff APPENDICES