Hemostasis and Thrombosis Protocols

Hemostasis.- Basic Techniques.- Isolation of DNA and RNA.- Amplification of DNA and RNA by PCR.- Direct Sequencing of PCR Products.- Solid-Phase Sequencing of Biotinylated PCR Products with Streptavidin-Coated Magnetic Beads.- Automated DNA Sequencing.- Detection of DNA by Silver Staining.- Promoter Studies in Hemostasis.- Methods of Mutational Analysis.- Detection of Mutations and Polymorphisms in Clotting Factors by Denaturing Gradient Gel Electrophoresis.- Screening for Mutations in DNA by Single-Stranded Conformation Polymorphism (SSCP) Analysis.- Screening for DNA Heteroduplexes in the Factor VII Gene Using Ethylene Glycol Gel Electrophoresis of Solvent-Treated 32P-Labeled PCR Products.- Detection of Mutations Causing Hemophilia A Using an In Vitro Coupled Transcription and Translation System.- Screening for Mutations in the Human Antithrombin Gene by Hydrolink D-5000™ and MDE™ Gel Electrophoresis.- Methods for Analyzing Inherited/Acquired Disorders of Hemostasis.- Detection of Mutations in Hemophilia A Patients by Chemical Cleavage of Mismatch Method.- Inversion Mutation Analysis in Hemophilia A by Restriction Enzyme Analysis and Southern Blotting.- Hemophilia B Mutational Analysis.- Screening for Candidate Mutations Causing von Willebrand’s Disease (vWD).- Use of Intron 40 VNTR I in vWD Gene Tracking.- Multimeric Analysis of von Willebrand Factor.- Identification of Mutations in the Human Factor VII Gene.- Molecular Analysis in Factor XI Deficiency.- Mutational Analysis in Antithrombin Deficiency.- Ectopic Transcript Analysis in Human Antithrombin Deficiency.- Mutational Analysis of the Human Protein C Gene.- Analysis of the Protein S Gene in Protein S Deficiency.- Screening for the G to A Transition at Position 20210 in the 3?-Untranslated Region (UTR)of the Prothrombin Gene.- Screening for the Factor V Leiden Mutation.- Multiplex PCR for Detection of the Prothrombin 3?-UTR (G20210A) Polymorphism and the Factor V Leiden Mutation.- Isoelectric Focusing and Immunodetection of Plasma Antithrombin.- Characterization of Heparin Binding Variants of Antithrombin by Crossed Immunoelectrophoresis in the Presence of Heparin.- The Determination of Amino Acid Sequence Abnormalities in Proteins by HPLC Peptide Analysis.- Platelet and Megakaryocyte Analysis.- Molecular Biological Identification and Characterization of Inherited Platelet Receptor Disorders.- In Vitro Expansion of Megakaryocytes from Peripheral Blood Hematopoietic Progenitors.- Molecular Biology Studies with Primary Megakaryocytes.