Protocols for Nucleic Acid Analysis by Nonradioactive Probes

Nucleic Acid Extractions.- Genomic DNA Isolation From Different Biological Materials.- Extraction of Plant RNA.- Indirect Detection.- Overview of Hybridization and Detection Techniques.- Checkerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection.- Nonradioactive Northern and Southern Analyses From Plant Samples.- Screening a BAC Library With Nonradioactive Overlapping Oligonucleotide (Overgo) Probes.- Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes.- In Situ Hybridization of Termite Microbes.- Fluorescent Labeling and Detection.- RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer.- Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization.- Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification.- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes.- Kinetic (“Real-Time”) PCR.- to Kinetic (Real-Time) PCR.- Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR.- Real-Time Quantitative PCR as an Alternative to Southern Blot or Fluorescence In Situ Hybridization for Detection of Gene Copy Number Changes.- Design and Work-Up of a New Molecular Diagnostic Assay Based on Real-Time PCR.- Real-Time PCR Fluorescent Chemistries.- Microarrays.- Microarrays.- Oligonucleotide Microarrays for the Study of Coastal Microbial Communities.