Adenovirus Methods and Protocols

Volume 2: Ad Proteins and RNA, Lifecycle and Host Interactions, and Phyologenetics

William S. M. Wold, Ann E. Tollefson (Herausgeber)

Buch | Softcover

362 Seiten

2010 | Softcover reprint of hardcover 2nd ed. 2007
Humana Press Inc. (Verlag)
978-1-61737-835-5 (ISBN)

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Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise.

Volume 1 addresses several important techniques for construction of adenoviruses for use as vectors and for basic research. Highlighted topics include deletion mutants, capsid modifications, insertions, and gene replacements in human, murine, bovine, and ovine adenoviruses. In Volume 2, the authors focus on methods that elucidate and quantitate the interactions of Ad with the host. Each of the protocols in these volumes provides a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.

Analysis of the Efficiency of Adenovirus Transcription.- The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domains.- Preparation of Soluble Extracts From Adenovirus-Infected Cells for Studies of RNA Splicing.- In Vitro Methods to Study RNA Interference During an Adenovirus Infection.- Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situ.- Study of Nucleolar Localization of Adenovirus Core Proteins.- Analysis of Adenovirus Infections in Synchronized Cells.- Co-Immunoprecipitation of Protein Complexes.- Chromatin Immunoprecipitation to Study the Binding of Proteins to the Adenovirus Genome In Vivo.- Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assays.- Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry.- The Use of Cell Microinjection for the In Vivo Analysis of Viral Transcriptional Regulatory Protein Domains.- Determination of the Transforming Activities of Adenovirus Oncogenes.- Human Adenovirus Type 12.- Measurement of Natural-Killer Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells.- A Flow Cytometric Assay for Analysis of Natural-Killer Cell-Mediated Cytolysis of Adenovirus-Transformed Cells.- Large-Scale Purification and Crystallization of Adenovirus Hexon.- Synthesis and Assay of Recombinant Adenovirus Protease.- Assay for the Adenovirus Proteinase.- Cofactors of the Adenovirus Proteinase.- Characterization of the Adenovirus Fiber Protein.- Phylogenetic Analysis of Adenovirus Sequences.- Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studies.

Erscheint lt. Verlag	10.11.2010
Reihe/Serie	Methods in Molecular Medicine ; 131
Zusatzinfo	54 Illustrations, black and white; XIV, 362 p. 54 illus.
Verlagsort	Totowa, NJ
Sprache	englisch
Maße	152 x 229 mm
Themenwelt	Medizin / Pharmazie ► Medizinische Fachgebiete ► Mikrobiologie / Infektologie / Reisemedizin
	Medizin / Pharmazie ► Medizinische Fachgebiete ► Onkologie
	Studium ► 2. Studienabschnitt (Klinik) ► Humangenetik
	Naturwissenschaften ► Biologie ► Mikrobiologie / Immunologie
	Naturwissenschaften ► Biologie ► Zellbiologie
ISBN-10	1-61737-835-6 / 1617378356
ISBN-13	978-1-61737-835-5 / 9781617378355
Zustand	Neuware