The Nuclear Receptor Superfamily

Nuclear Receptors: One Big Family.- Ligand Binding and Nuclear Receptor Turnover.- Methods for Measuring Ligand Dissociation and Nuclear Receptor Turnover in Whole Cells.- Flow Cytometry as a Tool for Measurement of Steroid Hormone Receptor Protein Expression in Leukocytes.- X-Ray Crystallography of Agonist/Antagonist-Bound Receptors.- Nuclear Localization and DNA Binding.- FRAP and FRET Methods to Study Nuclear Receptors in Living Cells.- Receptor-DNA Interactions: EMSA and Footprinting.- Chromatin Immunoprecipitation (ChIP) Methodology and Readouts.- Nuclear Receptor—Co-Regulatory Protein Interactions.- Yeast-Based Reporter Assays for the Functional Characterization of Cochaperone Interactions with Steroid Hormone Receptors.- High Throughput Analysis of Nuclear Receptor–Cofactor Interactions.- Binding Affinity and Kinetic Analysis of Nuclear Receptor/Co-Regulator Interactions Using Surface Plasmon Resonance.- Using RNA Interference to Study Protein Function.- Using Intrinsic Fluorescence Emission Spectroscopy to Study Steroid Receptor and Coactivator Protein Conformation Dynamics.- Pathophysiological Analysis of Nuclear Receptor Function.- Development of Phosphorylation Site-Specific Antibodies to Nuclear Receptors.- Tissue-Selective Knockouts of Steroid Receptors: A Novel Paradigm in the Study of Steroid Action.- Methods for Identifying and Studying Genetic Alterations in Hormone-Dependent Cancers.