Bacterial Virulence

Generation of Markerless Gene Deletion Mutants in Listeria monocytogenes Using a Mutated PheS for Counterselection.- A Rapid Fluorescence-Based Screen to Identify Regulators and Components of Interbacterial Competition Mechanisms in Bacteria.- Predicting Type III Effector Proteins Using the Effectidor Web-Server.- Assay for Type III Secretion in Escherichia coli.- Profiling of Secreted Type 3 Secretion System Substrates by Salmonella enterica.- Analysis of SPI-1 Dependent Type III Secretion and Injection Using a NanoLuc Luciferase-Based Assay.- Mycobacterium tuberculosis Infection of THP-1 Cells: A Model for High Content Analysis of Intracellular Growth and Drug Susceptibility.- Bone Marrow-Derived Macrophage (BMDM) Infection by Listeria monocytogenes.- Preparation and Inflammasome Activation of Murine Bone Marrow-Derived and Resident Peritoneal Macrophages.- Flow Cytometry-Based Single Cell Analyses of Bacterial Adaption to Intracellular Environments.- Quantification of Microbial Fluorescent Sensors during Live Intracellular Infections.- Dissecting Human Blood Immune Cells Response to Intracellular Infection Using Single Cell RNA-Sequencing.- Salmonella enterica Infection of Human and Mouse Colon Organoid-Derived Monolayers.- Analysis of Salmonella enterica Adhesion to Leaves of Corn Salad or Lettuce.- Methods for Using the Galleria mellonella Invertebrate Model to Probe Enterococcus faecalis Pathogenicity.- Murine Soft Tissue Infection Model to Study Group A Streptococcus (GAS) Pathogenesis in Necrotizing Fasciitis.- Mouse Model to Study Salmonella-Induced Colitis.- Analysis of Salmonella Typhi Pathogenesis in a Humanized Mouse Model.- In Vivo Tracking of Bacterial Colonization in Different Murine Models Using Bioluminescence: The Example of Salmonella.- Two In Vivo Models to Study Salmonella Asymptomatic Carrier State in Chicks.