NADPH Oxidases

Intersecting Stories of the Phagocyte NADPH Oxidase and Chronic Granulomatous Disease.- Mammalian NADPH Oxidases.- Enhanced Immunoaffinity Purification of Human Neutrophil Flavocytochrome B for Structure Determination by Electron Microscopy.- Purification and Characterization of DUOX Peroxidase Homology Domains (PHDs).- A Close-Up View of the Impact of Arachidonic Acid on the Phagocyte NADPH Oxidase.- NOX5 Cell-Free Assay for the High-Throughput Screening of Small Molecules.- Spectroscopy of NOX Protein Family Members.- Soluble Regulatory Proteins for Activation of NOX Family NADPH Oxidases.- Insights into the NOX NADPH Oxidases Using Heterologous Whole Cell Assays.- The X-CGD PLB-985 Cell Model for NOX2 Structure-Function Analysis.- Functional Characterization of DUOX Enzymes in Reconstituted Cell Models.- Guidelines for the Detection of NADPH Oxidases by Immunoblot and RT-qPCR.- Methods for Detection of NOX-Derived Superoxide Radical Anion and Hydrogen Peroxide in Cells.- HPLC-BasedMonitoring of Oxidation of Hydroethidine for Detection of NADPH Oxidase-Derived Superoxide Radical Anion.- Visualization of Intracellular Hydrogen Peroxide with the Genetically-Encoded Fluorescent Probe HyPer in NIH-3T3 Cells.- Imaging Intracellular H2O2 with the Genetically-Encoded PerFRET and OxyFRET Probes.- Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae.- Kinetic Analysis of Phagosomal ROS Generation.- Imaging Intestinal ROS in Homeostatic Conditions Using L-012.- Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo.- Phosphorylation of Gp91phox/NOX2 in Human Neutrophils.- The Molecular Regulation and Functional Roles of NOX5.- Using Synthetic Peptides for Exploring Protein-Protein Interactions in the Assembly of the NADPH Oxidase Complex.- Rational Design and Delivery of NOX-Inhibitory Peptides.- High-Throughput Screening of NOX Inhibitors.- Protein-Protein Interaction Assay to Analyze NOX4/p22phox Heterodimerization.- Isolation of Redox Active Endosomes (Redoxosomes) and Assessment of NOX Activity.- Model Systems to Investigate NOX-Dependent Cell Migration and Invasiveness.- NADPH Oxidases and Aging Models of Lung Fibrosis.- Proteomic Methods to Evaluate NOX-Mediated Redox Signaling.- Neutrophil Extracellular Traps.- Chronic Granulomatous Disease.- Diagnostic Testing for Chronic Granulomatous Disease.- Gastrointestinal Complications in Chronic Granulomatous Disease.- Ex Vivo Models of Chronic Granulomatous Disease.- Gene Editing in Chronic Granulomatous Disease.- DUOX Defects and Their Roles in Congenital Hypothyroidism.- NAPDH Oxidases in Inflammatory Bowel Disease.