Immunosenescence

Isolation of Lipid Rafts from Human Neutrophils by Density Gradient Centrifugation.- Flow Cytometry Analysis of NK Cell Phenotype and Function in Ageing.- Flow Cytometric Identification of Fibrocytes in the Human Circulation.- Experimental Approaches to Tissue Injury and Repair in Advanced Age.- Multicolor Digital Flow Cytometry Used in Human Translational Immunology.- Flow Cytometry-Based Methods to Characterize Immune Senescence in Nonhuman Primates.- Multiparameter Phenotyping of Human PBMCs Using Mass Cytometry.- Imaging Immunosenescence.- Activation-induced Cytidine Deaminase and Switched Memory B Cells as Predictors of Effective In Vivo Responses to the Influenza Vaccine.- Analyzing the Effects of Aging on CD8+ T Cell Phenotype Using Flow Cytometry.- Cell-Mediated Immune Response to Influenza Using Ex Vivo Stimulation and Assays of Cytokine and Granzyme B Responses.- Assays for Monitoring Macroautophagy Activity in T Cells.- Fluorescence-Based Approaches for Quantitative Assessment of Protein Carbonylation, Protein Disulfides and Protein Conformation in Biological Tissues.- Monitoring the DNA Damage Response at Dysfunctional Telomeres.- Single-Cell Analysis of T Cell Receptor ab Repertoire.- Assessment of B Cell Repertoire in Humans.- Laboratory and Data Analysis Methods for Characterization of Human B Cell Repertoires by High-throughput DNA Sequencing.- Discovery of Novel MicroRNAs in Aging C. Elegans.- Analysis of DNA Methylation by Pyrosequencing.