Fungal Secondary Metabolism

Library Preparation and Data Analysis Packages for Rapid Genome Sequencing.- Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters.- Media and Growth Conditions for Induction of Secondary Metabolite Production.- Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays.- The “FERMEX” Method for Metabolite-Enriched Fungal Extracts.- The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites.- Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri.- Production, Detection, and Purification of Clavine-Type Ergot Alkaloids.- Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS).- Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans.- Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis.- Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host.- Multi-Copy Genetic Screen in Aspergillus nidulans.- Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification.- Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra.- Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi.- Targeted Proteomics for Metabolic Pathway Optimization.- Investigation of In Vivo Protein Interactions in Aspergillus Spores.- Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus.- Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi.- Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects.