Methods of Animal Experimentation (eBook)

Front Cover 1
Methods of Animal Experimentation 4
Copyright Page 5
Table of Contents 10
LIST OF CONTRIBUTORS 6
PREFACE 8
CONTENTS OF VOLUME II 14
INTRODUCTION 16
Chapter 1. Collection and Withdrawal of Body Fluids and Infusion Techniques 18
I. Introduction 18
II. Collection and Withdrawal of Body Fluids 19
III. Infusion Techniques 49
References 57
Chapter 2. Anesthesia and Sedation 60
I. Introduction 61
II. Aims of an Ideal General Anesthetic 61
III. Objectives and Purposes of Different Types of Anesthesia 62
IV. Basic Principles 62
V. Methods of Preanesthesia 79
VI. Methods of Anesthesia 90
VII. The Use of Postoperative Analgesics and Sedatives 98
VIII. Emergency Measures for Experimental Animals Undergoing Procedures Requiring an Anesthetic Agent 99
IX. Preparation of Anesthesia of Individual Experimental Animals 102
X. Concluding Comments 117
References 118
Chapter 3. Care of Animals During Surgical Experiments 120
I. Introduction 120
II. Preoperative Care 121
III. Care During Operation 136
IV. Postoperative Care 155
References 165
Chapter 4. Radiography 168
I. Introduction 168
II. Principles of Radiographic Interpretation 169
III. Principles of Radiographic Technique 171
IV. Radiation Safety 179
V. Conclusion 180
References 180
Additional Bibliography 181
Chapter 5. Methods of Euthanasia and Disposal of Laboratory Animals 184
I. Introduction 184
II. Factors Involved in Euthanasia 188
III. Reasons for Euthanasia 190
IV. Procedures 191
V. Disposal 208
VI. Source Material 211
References 212
Chapter 6. Methods of Parasitic Infections: Outline of General Principles 214
I. Introduction 214
II. Cautions in the Choice of a Parasite and Animal Host 217
III. Factors Which Influence the Course of Infection with a Parasite 218
References 228
Chapter 7. Methods in Germfree Animal Research 232
I. Introduction 232
II. Germfree Animal Equipment 235
III. Securing and Maintaining Germfree Animals 243
IV. The Germfree Animal on Experiment 268
V. Some Information Derived from Germfree Animal Studies 274
VI. Outlook for the Future 283
References 284
Chapter 8. Aerosol Challenge of Animals 290
I. Introduction 291
II. Basic Aerosol Terminology 293
III. Aerosol Test Facilities and Systems 296
IV. Generation of Experimental Aerosols 302
V. Sampling Biological Aerosols 307
VI. Aerosol Challenge of Animals 313
VII. Maintenance of Aerosol-Challenged Animals 325
VIII. Diagnostic Procedures for Aerosol-Challenged Animals 339
IX. Laboratory Design Principles 347
X. Safety Management in Infectious Disease Laboratories 351
References 353
Chapter 9. Principles in Drug Administration 360
I. Introduction 360
II. Preparation of Animal 363
III. Preparation of Drug 366
IV. Gastrointestinal Routes 368
V. Parenteral Routes 369
VI. Inhalation Route 371
VII. Topical Route 373
VIII. Choice of Animal Species 374
IX. Experimental Design and Statistical Evaluation 375
X. Conclusion 375
References 376
Supplementary Bibliography 376
AUTHOR INDEX 378
SUBJECT INDEX 389