Crystalline Bacterial Cell Surface Proteins (eBook)

Front Cover 1
Crystalline Bacterial Cell Surface Proteins 2
Copyright Page 3
Contents 6
Chapter 1. Introduction 16
Chapter 2. Occurrence, Location, Ultrastructure and Morphogenesis of S-Layers 20
2.1. Introduction 20
2.2. Occurrence and Location of S-layers on Bacterial Cell Envelopes 21
2.3. Ultrastructure of S-layers 23
2.4. Morphogenesis of S-layers 28
2.5. Conclusions 37
Chapter 3. Chemical Composition and Biosynthesis of S-Layers 50
3.1. Introduction 50
3.2. Non-Glycosylated S-Layer Proteins 52
3.3. Glycosylated S-Layer Proteins 64
3.4. Conclusions 77
Chapter 4. Analysis of S-Layer Proteins and Genes 92
4.1. Introduction 92
4.2. Promoter Structures of S-Layer Genes 94
4.3. Secretion of S-Layer Proteins 99
4.4. Protein Homology Studies Among Different S-Layer Proteins and Related Proteins 100
4.5. S-Layer Protein Variations 103
4.6. S-Layer Domains 106
4.7. Recombinant S-Layer Genes and Molecular Biotechnology 109
Chapter 5. Functional Aspects of S-Layers 118
5.1. Introduction 118
5.2. Specific Functions 119
5.3. General Functional Aspects 128
5.4. Conclusion 136
Chapter 6. Biotechnological Applications of S-Layers 148
6.1. Introduction 148
6.2. S-Layer Ultrafiltration Membranes (SUMs) 149
6.3. Continuous Culture of S-Layer Carrying Organisms 151
6.4. S-Layers as Matrix for the Immobilization of Functional Macromolecules 153
6.5. S-Layer Coated Liposomes 165
6.6. Conclusion 168
Chapter 7. Vaccine Development Based on S-Layer Technology 176
7.1. Introduction 176
7.2. S-Layers as a Fish Vaccine 176
7.3. S-Layers as Carrier/Adjuvant for Vaccination and Immunotherapy 177
7.4. Conclusions 183
Chapter 8. Molecular Nanotechnology and Biomimetics with S-Layers 190
8.1. Introduction 190
8.2. Formation of S-Layer Lattices on Solid Substrates and Liquid Surface Interfaces 191
8.3. S-Layers as Patterning Structures and Nanonatural Resists in Molecular Nanotechnology 205
8.4. Biomimetic Applications 212
8.5. Conclusion 216
Appendix: Crystalline Surface Layers on Eubacteria and Archaeobacteria 226
Index 242