Molecular Biomethods Handbook (eBook)
XX, 1124 Seiten
Humana Press (Verlag)
978-1-60327-375-6 (ISBN)
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors' own research programs.
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up to date collection of methods used regularly in the authors' own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.
Molecular Biomethods Handbook 2nd Ed
Contents
PART A: NUCLEIC ACID BIOMETHODS
1 The manipulation of nucleic acids: basic tools and techniques – Gayle Corkill and
Ralph Rapley
2 Restriction Enzymes – Gareth J.S. Jenkins
3 Principles and Medical Applications of the Polymerase Chain Reaction – Birmal D.M.
Theophilus
4 Probe Design, Production, and Applications – Marilena Aquino de Muro
5 Southern Blotting as a Diagnostic Method – Bronwen Harvey and Pirkko Soundy
6 Introduction to Capillary Electrophoresis of DNA - Beatriz Sanchez-Vega
7 Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid
analysis - Kim Hung Leung and Shea Ping Yip
8 Denaturing Gradient Gel Electrophoresis (DGGE) – Jeroen H. Roelfsema and Dorien
J.M. Peters
9 Single Strand Conformation Polymorphism (SSCP) Analysis - Kim Hung Leung and
Shea Ping Yip
10 Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic
characterization of different organisms - Lúcia Maria da Cunha Galvão and Eliane
Lages-Silva
11 Quantification of mRNA using Real Time RT-PCR – the SYBR solution - David
Sugden and Patricia de Winter
12 Quantitative Analysis of DNA Sequences by PCR and Solid Phase Sequencing – Anu
Suomalainen and Ann-Christine Syvanen
13 MAPH (Multiplex Amplifiable Probe Hybridization) - Carolina Sismani, Ludmila
Kousoulidou and Philippos C. Patsalis
14 Gene Expression Profiling - Arnis Druka, Robbie Waugh and Pete Hedley
15 Comparative Genomic Hybridization in Clinical and Medical Research – Peng-Hui
Wang, Yann-Jang Chen and Chi-Hung Lin
16Subtractive Hybridization – Craig Winstanley
17 Fluorescence in situ Hybridization - Jane Bayani and Jeremy A. Squire
18 Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice -
Jonathan D. Smith
19 cDNA Microarrays – Phillip G. Febbo
20 Mapping Techniques – Simon G. Gregory
21 Single Nucleotide Polymorphisms Overview – Nameeta Shah
22 Bioinformatics Tools for Gene and Protein Sequence Analysis– Bernd H.A. Rehm and
Frank Reinecke
PART B: PROTEIN AND CELL METHODS
23. Protein Electrophoresis - David Sheehan and Siobhan O’Sullivan
24. Protein blotting - Patricia Gravel
25. Capillary electrophoresis of proteins - Mark Strege
26. Autoradiography and fluorography - Bronwen Harvey
27. Mass spectrometry of proteins & peptides - Kenneth G. Standing
28. Post-translational modifications - Christoph Kannicht and Birte Fuchs
29. Protein microarray technology - Charlotte H. Clarke and Eric T. Fung
30. Protein-protein interactions - Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith Wilkinson and Haian Fu
31. Glycoprotein analysis - Terry Butters and David C.A. Neville
32. Solid Phase Peptide Synthesis - Mare Cudic and Gregg B. Fields
33. Monoclonal antibodies - Zhong J. Zhang and Maher Albitar
34. Antibody Phage display - Rob Aitken
35. Protein engineering - Thomas Willemsen, Urs B. Hagemann,
Eva M. Jouaux, Sabine Stebel, Jody M. Mason, Kristian M. Müller and Katja M. Arndt
36. Directed protein evolution - Sabine C. Stebel, Annette Gaida, Katja M. Arndt and Kristian M. Müller
37. Enzyme Linked Immunosorbent Assay - John Crowther
38. Epitope mapping - Glenn E. Morris
39.
| Erscheint lt. Verlag | 4.11.2008 |
|---|---|
| Reihe/Serie | Springer Protocols Handbooks | Springer Protocols Handbooks |
| Zusatzinfo | XX, 1124 p. |
| Verlagsort | Totowa |
| Sprache | englisch |
| Themenwelt | Medizin / Pharmazie ► Studium |
| Naturwissenschaften ► Biologie ► Biochemie | |
| Naturwissenschaften ► Biologie ► Genetik / Molekularbiologie | |
| Technik | |
| Schlagworte | Bioinformatics • biomethods • Cryopreservation • DNA • electron microscopy • gene expression • genes • Hybridization • Molecular Biology • nucleic acid biomethods • Nucleotide • PCR • protein and cell biomethods • proteins • tissue |
| ISBN-10 | 1-60327-375-1 / 1603273751 |
| ISBN-13 | 978-1-60327-375-6 / 9781603273756 |
| Haben Sie eine Frage zum Produkt? |
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