Helicases

Helicases: An Overview.- Fluorescent Biosensors to Investigate Helicase Activity.- Single-Molecule FRET Analysis of Helicase Functions.- Kinetics of Motor Protein Translocation on Single-Stranded DNA.- Experimental and Computational Analysis of DNA Unwinding and Polymerization Kinetics.- Protein Displacement by Helicases.- In Vivo Investigation of Protein–Protein Interactions for Helicases Using Tandem Affinity Purification.- Mapping Genomic Targets of DNA Helicases by Chromatin Immunoprecipitation in Saccharomyces cerevisiae.- Methods to Study How Replication Fork Helicases Unwind DNA.- Simple Enzymatic Assays for the In Vitro Motor Activity of Transcription Termination Factor Rho from Escherichia coli.- Single-Molecule Studies of RecBCD.- Mutational Analysis of Bloom Helicase.- In Vivo Analysis of Drosophila BLM Helicase Function During DNA Double-Strand Gap Repair.- Purification and Characterization of RecQ Helicases of Plants.- Fluorometric Assay of Hepatitis C Virus NS3 Helicase Activity.- A Method to Simultaneously Monitor Hepatitis C Virus NS3 Helicase and Protease Activities.- Computer Modeling of Helicases Using Elastic Network Model.- Duplex Unwinding with DEAD-Box Proteins.- Analysis of the RNA Helicase p68 (Ddx5) as a Transcriptional Regulator.- A Method to Study the Role of DDX3 RNA Helicase in HIV-1.- Molecular Characterization of Nuclear DNA Helicase II (RNA Helicase A).- Regulation of Inter- and Intramolecular Interaction of RNA, DNA, and Proteins by MLE.- Biochemical Characterization of Human Upf1 Helicase.- Assays of the Helicase, ATPase, and Exoribonuclease Activities of the Yeast Mitochondrial Degradosome.- Characterization of the Helicase Activity and Anti-telomerase Properties of Yeast Pif1p In Vitro.- A Method to Confer Salinity Stress Toleranceto Plants by Helicase Overexpression.- A Method to Inhibit the Growth of Plasmodium falciparum by Double-Stranded RNA-Mediated Gene Silencing of Helicases.